Methods for Separating Sugars
Detection Methods (2)
When Is the Post-Column Fluorescence Method Necessary?
However, even though they are foods, what about brewed foods like soy sauce?
The typical sugar in soy sauce is glucose, which can be detected using a refractive index detector, but presumably those analyzing soy sauce are more interested in the other non-glucose sugars (such as ribose, mannose, arbinose, galactose, and xylose. If so, then it can no longer be handled with a refractive index detector. In terms of sensitivity, an electrochemical detector could be considered, but soy sauce contains large amounts of amino acids, which could interfere with analysis. In such cases, the post-column fluorescence method is required.
What Is the Post-Column Method Using Arginine Reagent?
This method can be used with each method for separating sugars described in LCtalk 49, but it is most effective when combined with the borate complex anion exchange method. The borate complex anion exchange method offers superior separation of disaccharides from monosaccharides. To improve separation efficiency, however, gradient elution is required, which precludes using a refractive index detector. This post-column fluorescence detection using arginine reagent combined with the borate complex anion exchange method is currently still widely used.
What Are the Advantages of the Arginine Reagent?
Furthermore, the arginine method enables detection of sucrose, which is a non-reducing disaccharide. Though the detection sensitivity is only one-tenth compared to the reducing disaccharide, maltose, the fact that it can detect sucrose is a significant advantage.
More About Arginine Reagent
Based on our study results, we determined that arginine in the mobile phase had no effect on sugar separation using the borate complex anion exchange method and reacted efficiently when heated to 150 °C in the reaction coil.
What About Analyzing Soy Sauce?
2) H.Mikami and Y.Ishida: 10th International Symposium on Column Liquid Chromatography (1986).
For Research Use Only. Not for use in diagnostic procedures.
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