Qualitative Analysis of Alkaloids Contained in Coptis Rhizome – LCMS

Crude Drugs

Qualitative Analysis of Alkaloids Contained in Coptis Rhizome – LCMS

Coptis rhizome has long been utilized as a bitter-tasting digestive aid. Even today, it is a crude drug included in digestive medicines. Berberine and other isoquinoline alkaloids, which are the active ingredients in coptis rhizome, can be analyzed with an HPLC system using ODS columns.
However, since the basis for identifying each peak is retention time, it is necessary to check the results using berberine, coptisine, palmatine and other standard samples. Furthermore, each component has a similar absorption spectrum, so they must be qualified very carefully.
We analyzed the alkaloids (berberine, epiberberine, coptisine, jatrorrhizine, and palmatine) in powdered coptis rhizome using LCMS, from which the mass spectra were obtained in addition to retention times.


The mass chromatogram shown is derived from the mass numbers for the protonated molecules of each component.
We confirmed the separation of each component, thereby identifying each component.

Mass Chromatogram for Alkaloids in Coptis Rhizome

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Pharmaceutical, Life Science