Microorganism Identification System

Provides better discrimination capabilities

Strain Solution (option)

Highly-accurate Bacterial Characterization Using Fast and Simple MALDI-TOF MS

Based on proteotyping techniques using a MALDI-TOF MS mass spectrometer, Strain Solution is intended for (1) characterization of bacteria at the subspecies level (such as characterization of subspecies / strain level and screening for specific serotypes & genotypes) and (2) molecular phylogenetic analysis for research use.
Using this approach, preregistered protein markers are used to differentiate the subspecies and strain level of bacteria. The Strain Solution software determines whether or not this data matches the acquired MALDI-TOF MS data for target bacterial strains.
  • This instrument is not available in the USA and may not be available in some other countries. Please contact your local Shimadzu representative for availability.

Features

Difference in Marker Peaks for E. coli O157 and non-O157 Bacteria*
* Teruyo Ojima-Kato, Naomi Yamamoto, Mayumi Suzuki, Tomohiro Fukunaga, and Hiroto Tamura: Discrimination of Escherichia coli O157, O26 and O111 from other serovars by MALDI-TOF MS based on the S10-GERMS method. PLoS ONE, 2014, 9: e113458.

 

  • Obtain more accurate and reliable characterization results than conventional microorganism differentiation methods using MALDI-TOF MS fingerprinting.
  • Quickly characterize bacteria by differences in the genetic sequence using MALDI-TOF MS.
  • Perform molecular phylogenetic analysis on a theoretical basis using proteotyping techniques in combination with the cluster analysis functionality included in the software.
  • Allows for the creation of new/custom protein marker databases.
 
 
 
 

Applications

The software broadens the range of possible applications using the iDplus series by enabling bacterial characterization below species level.
This research-use software is ideally suited for:
 
  • Management of bacterial culture collections
  • Discrimination of genetically similar bacteria that are difficult to differentiate by 16S rRNA gene sequencing
  • Molecular phylogenetic analysis.
  • Serotypic screening
Proteotyping
Proteotyping involves using assigned protein components detected using a MALDI-TOF MS system as markers for characterizing microorganisms. The markers are identified in advance, for example, using genetic sequence information. Compared to conventional gene-based typing methods, such as DNA sequencing, this offers the advantage of a faster and simpler method. If the genomic sequence for the target bacteria species has not been decoded, proteotyping can be accomplished by decoding the base sequence for the S10-spc-alpha operon, for which about half of the ribosomal proteins have been coded (S10-GERMS method).

Workflow Using Strain Solution Ver. 2

 

Precautions

*1 Steps indicated inside the blue box (top left) are not included in the software functionality.
*2 The standard configuration of the software does not include a marker peak database. To use the system, bacterial strain marker peaks for target bacterial species must be registered in a database in advance. If the dedicated database (optional) is used, the step for searching marker peaks can be omitted.

Strain Solution Dedicated Database (Optional)

 
 
List of Registered Bacterial Species
Genus Species Serotype
 Escherichia  coli  O157
 O26
 O111
 
 albertii  
 Listeria  monocytogenes  1/2a
 1/2b
 1/2c
 3a
 3b
 3c
 4a
 4b
 4d
 nnocua  6a
 
 ivanovii subsp. ivanovii  
 ivanovii subsp. londoniensis  
 seeligeli  
 welshimeri  
 

Precautions

  1. This database is optional. The list of registered bacterial species is current as of September 2016.
  2. Shimadzu makes no warranty regarding the accuracy of information included in the product or the usefulness of information obtained from using the product.

Strain Solution software was created on the basis of the results obtained from the Food Safety & Security Technology Development Project (group leader: Professor Hiroto Tamura of the Laboratory of Environmental Microbiology, Faculty of Agriculture, Meijo University), a "Knowledge Hub Aichi" core research project of Aichi Prefecture, which is in-turn based on ideas from the Meijo University and the National Institute of Advanced Industrial Science and Technology.
 
 

For Research Use Only. Not for use in diagnostic procedures.

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