PPSQ-51A/53A
Protein Sequencer

Easier and More Reliable Amino Acid Sequencing
Support for Data Integrity Compliance
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Protein Sequencer
Edman degradation is used to successively cleave one amino acid (PTH-amino acid) at a time from the N-terminus of a protein, each of which is then analyzed by HPLC to determine the amino acid sequence.
This diagram illustrates the process from Edman degradation to HPLC analysis of PTH-amino acids. This process is referred to as one cycle. By repeating this cycle and arranging the PTH–amino acids in the order they are obtained, the amino acid sequence of proteins and peptides can be easily and reliably determined. This process can also determine protein amino acid sequences not registered in a database. Therefore, in addition to basic research, the system can be used for a variety of other applications, such as analyzing proteins and peptides in foods or antibody therapeutics.
Analysis System Lineup for Various Applications
Two elution methods, isocratic and gradient, can be selected.
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Isocratic System
Easy to Use and Ideal for Routine Analysis
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Gradient System
Excellent Sensitivity Enables Analysis of Small Sample Quantities
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Features
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Uniquely increased PTH-amino acids to be easily identified.
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First-time users easily determine the amino acid sequences of proteins and peptides.
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The membrane holder kit allows for quick and easy sample preparation, including concentration and desalting, directly on a PVDF membrane.
News / Events
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"What Is Microchip Electrophoresis?" Now Available
Microchip electrophoresis uses a quartz substrate (microchip) with integrated channels and electrodes to separate DNA and RNA by nucleotide size.
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Release of PPSQ-51A/53A - Protein Sequencer
Shimadzu Corporation released the PPSQ-51A/53A SPD-40 model protein sequencers. These instruments can be used to analyze the amino acid sequences in proteins, such as for quality control of antibody drugs or peptide drugs and has " suitable for routine operations " and " offer accurate and reliable analytical performance.".
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Release of CellTune Ver.2 — AI Proposes Optimal Cell Culture Conditions
CellTune Ver.2 streamlines the optimization of complex cell culture conditions using statistical analysis and AI.
It helps achieve both cost reduction and improved quality in bioproduction. -
CASE STUDIES: "Lipid research at UT Southwestern could revolutionize diabetes treatment" is now available.
Can the science of fats unlock a new avenue in diabetes care? UT Southwestern researchers are finding out.
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New IMAGEREVEAL MS, Mass Spectrometry Imaging Data Analysis Software
Automatically uncover important information from large amounts of data with IMAGEREVEAL MS.
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New iMScope QT
Inheriting the concept of a mass spectrometer equipped with an optical microscope from the iMScope series, the iMScope QT is also Shimadzu's flagship model for MS imaging with a LCMS-Q-TOF.

