Determination of Storage Lipid Contents by Total Organic Carbon (TOC) Analyzer and Fourier Transform Infrared Spectrophotometer (FT-IR) in Non-Destructive Microalgal Cells

Recent progress in our understanding of biofuel production has increased the demand for the development of various technical methods for the quantification of biofuel candidates such as neutral lipids and polar lipids such as fatty acids. The keys factors required for increasing biofuel and biorefinery production are biological, particularly how to isolate highly productive microalgal strains and technological and engineering systems to establish highly energy-efficient high-cell-density-mass cultivation. For establishing such systems, the development of analytical methods which are useful for easy and rapid quantification of biofuel compounds is necessary.
FT-IR is very useful for getting relative quantification data of organic cellular components such as proteins, carbohydrates and lipids in non-disrupted cells. On the other hand,TOC analyzer can be used for the quantification of total organic and inorganic carbons in samples. Therefore, the combination of both FT-IR and TOC analysis was expected theoretically to enable us to get quantities of individual cellular organic components. However, it is impossible to quantify all cellular organic components by FT-IR although it does provide relative amounts of most major cellular organic components. Therefore, we sought to develop a new method to get “semi-quantitative values” of major cellular components.

Content Type:
Application
Document Number:
LAAN-C-XX-E026
Product Type:
Molecular Spectroscopy, Total Organic Carbon Analysis, FTIR Spectroscopy, TOC Analysis
Keywords:
Energy, Biofuel, Haptophyte, Alkenones, Namely Logng-Chain Ketones, Emiliania huxleyi, Biomass, Microalgae, Pleurochrysis carterae, P.carterae, Tisochrysis lutea, T.lutea, CCMP463, Chrysotila lamellosa, CCMP1307, CCMP2090, New Energy, IRAffinity-1S, TOC-LCPH
Language:
English
File Name:
jpa217014.pdf
File Size:
2,245kb

Free Download

For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

Top of This Page