Determination of Fatty-acid Composition of Lipids in Human Embryonic Stem Cells Using GC-MS
The cellular lipids were extracted from frozen pellets of human ES (embryonic stem) cells, and the constituent fatty acids were esterified to form fatty acid methyl esters. GC/MS analysis of the methylated fatty acids resulted in identification of 19 types of fatty acids.
This report introduces the pretreatment protocol used to extract the lipids from the cells, and the fatty acids that can be analyzed by this process.
- Content Type:
- Application
- Document Number:
- C146-E243
- Product Type:
- Mass Spectrometry, Gas Chromatograph-Mass Spectrometry
- Keywords:
- human ES cells, fatty acids, gas chromatography, quadrupole mass spectrometer, GC/MS, Pharmaceutical, Life Science, DMPK, ADME, Safety testing, GCMS-QP2010 Ultra
- Language:
- English
- File Name:
- jpo213109.pdf
- File Size:
- 487kb
