On-line LC-GCxGC-MS/MS: A Powerful Unified Separation-science Tool

This Technical Report demonstrates the potential of the on-line combination of high performance liquid chromatography (HPLC), cryogenically modulated comprehensive two-dimensional gas chromatography (GCxGC), and triple quadrupole mass spectrometry (MS/MS). The selectivity of the HPLC dimension enabled the separation of chemical classes; each fraction, transferred to the GCxGC instrument via a syringe-based interface, was subjected to a specific programmed temperature vaporizer (PTV) GCxGC-MS/MS untargeted or targeted analysis.