Multicomponent Analysis of Metabolites in Human Plasma using GC-MS/MS

The analysis of metabolomes, such as when searching for disease biomarkers, is performed in many areas in the medical field, whether it be for fundamental research or for clinical studies. Single quadrupole GC-MS provides excellent chromatographic resolution and enables stable measurements, and is therefore widely utilized for metabolome analyses involving the comprehensive analysis of in vivo metabolites. However, biological samples contain many metabolites and various matrices, so separation with single quadrupole GC-MS can be difficult. With triple quadrupole GC-MS/MS MRM, MS separation is performed twice, with Q1 and Q3. This helps remove the impact of overlapping peaks due to interfering components in comparison with scan mode and SIM mode, in which MS separation is performed with a single quadrupole, and thus enables the acquisition of accurate quantitative results with high-sensitivity detection.
Smart Metabolites Database registers MRM information of 475 metabolites mainly contained in biological samples such as blood, urine and cells. It enables simultaneous measurement of 475 metabolites using MRM mode. This application data sheet presents an analysis of metabolites in standard human plasma using the scan and MRM methods included in the Smart Metabolites Database, as well as a comparison of the results.