Comprehensive 2D GC with Dual Mass Spectrometry / Flame Ionization Detection for the Analysis of the Milk Unsaponifiable Lipid Fraction

A comprehensive two-dimensional GC (GCxGC) method, with dual FID/MS detection was developed for the qualitative and quantitative analysis of the entire unsaponifiable fraction of lipids belonging to various milk-types. The GCxGC column set used consisted of a low-polarity first dimension, and a medium-polarity secondary one, both characterized by a high thermal stability. The use of dual detection enabled the simultaneous attainment of both qualitative (mass spectral information) and quantitative data (%). The complexity of the fingerprint generated fully justified the employment of the two-dimensional GC technology, whose major valuable benefits were both sensitivity enhancement and the formation of group-type patterns.