Application for Plant Metabolome Analysis Using the GC/MS/MS Smart Metabolites Database

The GC/MS/MS Smart Metabolites Database contains analytical conditions for the high-sensitivity detection of 475 metabolites commonly involved in the central metabolism of all biological organisms.
Using this database, 170 metabolites, including amino acids, organic acids, and sugars, were detected in mature tomato leaves. Furthermore, by verifying the analytical accuracy of an internal standard substance, ribitol, based on the relative elution time, detection intensity, and reference ion ratio, we confirmed that it is possible to quantitatively detect metabolites with a high level of accuracy.