Semi-Quantitation of Toxicological Substances in Whole Blood Using the Quick-DB Forensic

The sample preparation is an indispensable process in GC-MS analysis. The recovery rate of sample preparation must be considered when quantifying target compounds in samples. However, the experience and time needed to determine the optimal sample preparation method and recovery rate increase with the number of compounds targeted for measurement. In addition, it is costly and difficult to acquire standard samples for quantitation and create calibration curves.The Quick-DB GC/MS/MS Forensic Toxicology Database (Quick-DB Forensic) contains the GC/MS/MS analytical conditions for 68 toxicological substances often involved in poisonings, as well as calibration curve information for multiple points throughout the sample preparation. This enables standard-less semi-quantitation of toxicological substances in whole blood samples using pretreatment in accordance with the optimized QuEChERS protocol.This application data sheet introduces an evaluation of the semi-quantitative accuracy by calculating quantitative results for a pretreated sample of whole blood with drugs added, using calibration curves registered in Quick-DB Forensic.