New Data Processing Method for Photodiode Array Detector
A new data processing method for a photo diode array (PDA) detector, Intelligent Dynamic Range Extension Calculator (i-DReC) enables the automatic calculation of peak area and height, utilizing spectrum similarity in the high concentration range where UV signal is saturated. When the integrated chromatographic peak area exceeds a user-defined threshold value, i-DReC automatically shifts the chromatographic profile to a wavelength with less UV absorption to prevent signal saturation. The absorption ratio between the original target wavelength and the wavelength used by the i-DReC function is applied as a correction factor to the peak area of the acquired chromatogram, thereby calculating the peak area and height at the original target wavelength. The i-DReC dramatically extends the linear dynamic range of calibration curves, enabling reliable quantitation of high concentration samples without need for sample dilution and reinjection, which would otherwise be required.
- Content Type:
- Application
- Document Number:
- C190-E167
- Product Type:
- Liquid Chromatography
- Keywords:
- PDA data processing, dynamic range extension, Nexera X2, UHPLC, Pharmaceutical, Life Science, DMPK, ADME, Safety testing, LC-30AD
- Language:
- English
- File Name:
- jpl213020.pdf
- File Size:
- 569kb