On-line Comprehensive RP-LCxRP-LC/IT-TOF for the Analysis of Proteome Isoforms
This bottom-up approach relies on both the power of 2D-LC separation techniques, and the sensitivity of MS (ESI-IT-TOF) detection. After separation by RP-LCxRP-LC is performed at the peptide level, both PDA and MS 2D plots are obtained by means of a dedicated software, which further allows qualitative and quantitative data analysis, as well as spectral comparison/subtraction. Tandem MS data obtained by collision-induced dissociation (CID) of the peptides were used for database search for α-casein and dephosphorylated α-casein, with high sequence coverage.
- Content Type:
- Application
- Document Number:
- C190-E176
- Product Type:
- Mass Spectrometry, Gas Chromatography, Liquid Chromatograph-Mass Spectrometry
- Keywords:
- Comprehensive RP-LCxRP-LC/PDA/IT-TOF, Tryptic digest, Proteome analysis, Protein isoforms, Pharmaceutical, Life Science, Electronics, Electronic, Proteomics, RP-LCxRP-LC/IT-TOF
- Language:
- English
- File Name:
- jpl214013.pdf
- File Size:
- 507kb