On-line Comprehensive RP-LCxRP-LC/IT-TOF for the Analysis of Proteome Isoforms

This bottom-up approach relies on both the power of 2D-LC separation techniques, and the sensitivity of MS (ESI-IT-TOF) detection. After separation by RP-LCxRP-LC is performed at the peptide level, both PDA and MS 2D plots are obtained by means of a dedicated software, which further allows qualitative and quantitative data analysis, as well as spectral comparison/subtraction. Tandem MS data obtained by collision-induced dissociation (CID) of the peptides were used for database search for α-casein and dephosphorylated α-casein, with high sequence coverage.

Content Type:
Application
Document Number:
C190-E176
Product Type:
Mass Spectrometry, Gas Chromatography, Liquid Chromatograph-Mass Spectrometry
Keywords:
Comprehensive RP-LCxRP-LC/PDA/IT-TOF, Tryptic digest, Proteome analysis, Protein isoforms, Pharmaceutical, Life Science, Electronics, Electronic, Proteomics, RP-LCxRP-LC/IT-TOF
Language:
English
File Name:
jpl214013.pdf
File Size:
507kb

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For Research Use Only. Not for use in diagnostic procedures.

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