Comprehensive Two-dimensional Liquid Chromatography for Determination of Polyphenols in Red Wines
A comprehensive two-dimensional liquid chromatography method was developed and applied to the determination of polyphenols in red wines. To fulfil such a task, a micro cyano and a partially porous octadecylsilane columns were employed in the first and the second dimension, respectively in combination with photodiode array and mass spectrometry (LCxLC-PDA-MS) detection. To increase the peak capacity values by using RP modes in both dimensions, a comparison of a conventional full-in-fraction and shifted second dimension gradient was carried out. The separation capabilities of the comprehensive LC approaches tested allowed the analysis of such a complex natural sample without any pre-treatment to effectively reduce the interferences coming from the matrix.
- Content Type:
- Application
- Document Number:
- C190-E178
- Product Type:
- Mass Spectrometry, Liquid Chromatograph-Mass Spectrometry
- Keywords:
- Comprehensive LC, Polyphenols, Red wines, Food and Beverages, Food QAQC, LCxLC-PDA-MS
- Language:
- English
- File Name:
- jpl214015.pdf
- File Size:
- 636kb