Simultaneous Analysis of Water-Soluble Vitamins by Newly Developed "Shim-pack MAqC-ODS I" Column
Water-soluble vitamins consist mainly of highly polar basic components which exhibit weak retention in analysis by reversed-phase liquid chromatography. Since they exhibit weak retention, the analysis is typically conducted using ion-pair reagents. Using ion-pair reagents can be challenging because of the longer analysis time, widening of slow eluting peaks, difficulty in obtaining sufficient sensitivity and long equilibration times. Analysis efficiency is also compromised due to additional work-flow requirements, such as mobile phase preparation and column conditioning. Here, we introduce an example in which the newly developed "Shim-pack MAqC-ODS I" column is used to conduct simultaneous analysis of water-soluble vitamins without the addition of ion-pair reagents to the mobile phase.
- Content Type:
- Application
- Document Number:
- LAAN-A-LC-E236
- Product Type:
- Liquid Chromatography
- Keywords:
- C18, Alkyl sulfonate, Water-soluble vitamins, Thiamine, Niacin, Nicotinamide, Pyridoxine, Ca Pantothenate, Folic acid, Riboflavin, Biotin, Food and Beverages, Pharmaceutical, Life Science, Shim-pack MAqC-ODS I, VP-ODS, SPD-M20A, Prominence
- Language:
- English
- File Name:
- jpl214024.pdf
- File Size:
- 1,002kb