Analysis of Kakkonto by Nexera-e and SPD-M30A Photodiode Array Detector (Part 1)

Kakkonto (traditional Chinese medicine) is believed to be effective against cold, headache, stiff shoulders, etc. because it has the properties to promote sweating and provide anti-inflammatory and analgesic action. It consists of puerariae radix, ephedra, licorice, paeoniae radix, etc. It contains many compounds such as ephedrine, glycyrrhizic acid, and cinnamic acid. The Nexera-e comprehensive two-dimensional liquid chromatograph is useful to separate such a complex sample.
Here, we tried a comprehensive separation by using a semi-micro reversed-phase column and neutral pH mobile phase for 1st dimension and a ultra-high speed reversed-phase column and acidic pH mobile phase for 2nd dimension. Glycyrrhizic acid was quantitated.