HPLC method scouting system using ultra high performance liquid chromatography coupled to single quadrupole mass spectrometer

Traditionally, the process of LC/MS amenable separation method development has been time consuming as well as labor intensive task. UHPLC based method scouting system will dramatically shorten total run time comparing to the conventional system and enables to output multiple results based on the various combination of different mobile phase and multiple columns allow you to make the comprehensive investigation of analytical conditions. And also, it is well known the sensitivity of mass spectrometric detection will be varied with the type of mobile phase. In this study, the UHPLC based method scouting system coupled to single quadrupole mass spectrometer was applied to develop the LC/MS amenable method for the simultaneous analysis of geno-toxic impurities, several amines and acidic compounds.