Rapid Analysis of Immuno-suppressants Using Triple Quadrupole LC-MS/MS

This report exemplifies an ultra high speed analysis of 4 immuno-supressants (Tacrolimus, Everolimus, Rapamycin, Cyclosporin A) in less than 120 seconds by Shimadzu UFMS Triple Quadrupole Mass Spectrometer LCMS-8050 with negative ionization mode. Combination of Nexera X2 and LCMS-8050 provides you much faster run time than ever you experienced without sacrificing the quality of results.

Including two internal standrads such as Ascomycin and Cyclosporin D, total 6 compounds were measured in 1.8 minutes. The structure of each compounds is illustrated below. In spite of low injection volume (1.5 uL) was utilized, this ultra high speed method achieves 1 ng / mL as the limit of quantitation for all compounds.