Simultaneous Analysis of 97 Primary Metabolites By PFPP: Pentafluorophenylpropyl Column

Energy production is essential for every living organism. Energy production at the cellular level is carried out by various metabolic processes, including the glycolytic system and the TCA cycle. In order to investigate the metabolism of living systems, it is important to quantify the amount of each metabolite. However, many primary metabolites are hydrophilic and difficult to analyze by reversedphase chromatography. Ion pair chromatography is sometimes used for the analysis of such hydrophilic compounds but it is not preferable for LCMS analysis because ion pair reagents cause high background signals and sensitivity deterioration.

A new method using a Pentafluorophenylpropyl (PFPP) column has been developed to overcome these limitations. This data sheet presents the simultaneous analysis of 97 primary metabolites, such as amino acids, organic acids, nucleotides, nucleosides and co-enzymes, using a PFPP column, which uses particles functionalized with a pentafluorophenylpropyl group.