Multi-residue pesticides analysis by LC-MS/MS using the ODS column and the biphenyl column

More than a thousand different types of pesticides are being used worldwide. In recent years, due to increased awareness of food safety, the amount of pesticide residues in food are controlled by local regulatory agencies that define the maximum residue levels (MRLs) and enforce compliance by regular inspection. LC-MS/MS is widely employed as an analytical method for pesticide residues in the food safety because it features excellent selectivity and sensitivity even in the matrix-matched samples. As for the separation with LC, ODS has long been the first choice column chemistry but biphenyl columns are emerging as an alternative.
Here we performed simultaneous LC-MS/MS analysis of 167 pesticides using both ODS and biphenyl columns for comparison.