A Fully Automated, Bottom-up Approach for MALDI-TOF MS Based Discovery Workflows

Bottom-up workflows have been a staple of mass spectrometry based proteomic approaches. Most of these protocols require overnight digestion, sample clean-up and, when involving MALDI, fraction collection, dry down and sample matrix deposition. Such lengthy protocols limit the number of samples that can be analyzed while suffering from great variability in digestion efficiency qualitatively and quantitatively. We present in this work a fully automated solution for MALDI-TOF MS based peptide mapping experiments. Using an online digestion, desalt, reversed phase separation and fraction collection platform, we were able to decrease the experimental time from over 18 hours to less than 30 min (reversed phase separation included . acquisition time not included) while substantially improving sequence coverage.

Content Type:
Paper
Document Number:
PO-CON1567E
Product Type:
Mass Spectrometry, Life Science Lab Instruments, MALDI-TOF Mass Spectrometry
Keywords:
Bottom-up, peptide mapping, Pharmaceutical, Life Science, Proteomics, MALDI-TOF MS
Language:
English
File Name:
sio315085.pdf
File Size:
608kb

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For Research Use Only. Not for use in diagnostic procedures.

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