Relative Quantum Yield Measurement of a Sample in Solution

Quantum yield is the ratio of the number of photons of excitation light adsorbed by a fluorescent material to the number of photons emitted by that material as fluorescent light. Quantum yield is used to evaluate the luminous intensity and luminous efficiency of fluorescent materials used in lighting and displays, and of fluorescent probes used in life sciences. Quantum yield can be calculated for unknown samples by a relative method that uses a fluorescent material of known quantum yield as a standard sample, or by an absolute method that uses an integrating sphere to calculate quantum yield directly (values calculated by the absolute method are called absolute quantum yield or quantum efficiency).
We describe using the RF-6000 spectrofluorophotometer to measure and calculate quantum yield by the relative method.

Content Type:
Application
Document Number:
LAAN-A-RF-E008
Product Type:
Molecular Spectroscopy, Fluorescence Spectroscopy
Keywords:
Relative quantum yield, Fluorescent material, Fluorescent probe, Fluorescent pigment, Rhodamine B, Uranine, RF-6000, LabSolutions RF, Quantum yield program, Electronics, Electronic, Hydrocarbon Processing Industry (Petrochemical, Chemical), Pharmaceutical, Life Science, Dye, Pigment, Paint, Ink, Printing, RF-6000
Language:
English
File Name:
jpa416012.pdf
File Size:
1,115kb

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