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NBS Biomarker search system
The NBS reagent kit is used for comprehensive quantitative analysis of proteins (biomarkers) typical to specific diseases in blood, cells or tissues. It is ideal for research involving high efficiency biomarker searches. Analyzing samples treated with NBS reagent using HPLC, AccuSpot, or AXIMA®-TOF2 in combination with quantitative proteome analysis support software enables high efficiency searches and the identification of intended proteins.
System Features
- High sensitivity analysis is enabled by using a new matrix selective for the NBS reagent.
- Efficient, highly reliable analysis is enabled by narrowing down the results at the MS stage.
- High accuracy protein identification is achieved thanks to high-sensitivity MS/MS analysis using the new AXIMA®-Performance mass spectrometer.
- The new analysis software (TWIP) is used to calculate relative quantities for all detectable doublet peaks, enabling easy comparative verification of the original data.
Biomarker candidate protein identification
13CNBS® Stable Isotope Labelling Kit-N
Stable isotope labeling kit for quantitative
proteome analysis
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NBS reagent
13CNBS® Stable Isotope Labelling Kit-N -
Protein samples extracted from pairs of differing (normal versus diseased) cells/tissues undergo stable isotope labeling with light reagent and heavy reagent respectively.
Prominence + AccuSpot
Automated spotting instruments for
HPLC and MALDI plates
- Prominence + AccuSpot
- The many peptides labeled by the NBS reagent are separated using HPLC. In addition, by using AccuSpot, eluate from the HPLC can be spotted directly on the MALDI plate, enabling fractionation.
AXIMA®-Performance
MALDI-TOF-TOF mass spectrometer
AXIMA®-Performance
MS measurements are performed in automatic measurement mode of the sample loaded on the 384 well MALDI plate.
TWIP
Quantitative proteome analysis
support software
TWIP*
Quantitative analysis is performed from the MS data obtained. Peaks of interest are then selected (such as peaks with highly variable width).
*This product was developed in cooperation with Dynacom Co., Ltd.
AXIMA®-Performance
MALDI-TOF-TOF mass spectrometer
AXIMA®-Performance
MS/MS analysis is performed for peaks selected with TWIP.Target proteins are then identified from a Mascot search (MS/MS Ion Search).
* The appearance and specifications are subject to changes for improvements without notice.
