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Detection of Genes Related to Food Poisoning Using an MCE-202 MultiNA Microchip Electrophoresis System

Over the past few years, genetic detection methods have become widely adopted for the identification of the causative agents of food poisoning, allergies, and infectious diseases such as influenza. These detection methods must be fast and highly sensitive. PCR amplification products have been conventionally detected using gel electrophoresis. The gel image after electrophoresis is evaluated visually but this method suffers disadvantages such as ambiguity when detecting trace levels. This page introduces the analysis of genes related to food poisoning using the MCE-202 MultiNA Microchip Electrophoresis System for DNA/RNA Analysis. This system offers higher detection sensitivity than agarose gel electrophoresis and allows simple comparison of large volumes of data.

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<Analysis Results>

Detection was possible in the target region for the ten types of genes related to food poisoning. Results of analysis using the MCE-202 MultiNA were obtained as electrophoresis images and electropherograms. Estimated size and concentration values for the amplification products are calculated from the calibration curve for the standard sample (ladder) and expressed as numeric values, allowing simple evaluation of the target amplification products.