Product

Laboratory Instruments

Spectroscopy Products

UV-VIS Spectrophotometers(UV-VIS)

UV-2450,UV-2550

UV2450,UV2550

Following their introduction in 1995, the world's highest performance, easy-to-use Shimadzu UV-2401/2501 spectrophotometers are now even more powerful with the addition of the world's most advanced software. These spectrophotometers have made their entrance to greet the new century.

Low stray light

The UV-2550, with its DDM (double blazed grating, double monochromator) design, provides a high energy throughput optical system with ultra low stray light. The UV-2450, the single monochromator version of the UV-2550, achieves 0.015% stray light even with its simpler optical system.

High Performance

Both instruments offer outstanding cost performance in their ability to provide UV-VIS spectral analysis of wide ranging samples, including organic and inorganic compounds, biological samples like DNA and enzymes, as well as special measurements on optical materials.

Windows XP Pro/2000 Pro Compatible

Operating under the Windows XP Pro/2000 Pro environment, the new generation UV Probe software is provided as standard. UVProbe incorporates the latest multitasking and report generation functionality, including network support, enabling measurement assistance among colleagues and effective presentation of precious data.

Analysis of multiple trace samples

The 8/16 micromulti-cell permits selection among 4 possible combinations with sample capacities of 50mL and 100mL. And, either the normal type or the constant-temperature controlled type cell holder may be selected.

（NOTE) Both the cell holder and the multi-cell are optional accessories.

UV-2450 and UV-2550 Specifications

Item
Description
Setting wavelength range
190 ~ 1100nm
Measurement wavelength range
190 ~ 900nm (up to 1100nm with special detector)
Wavelength accuracy
Ẕ0.3nm with auto wavelength correction included
Wavelength repeatability
Ẕ0.1nm
Wavelength scanning speed
Wavelength slew rate: about 3200nm/min
Wavelength scan rate: about 900 ~ 160nm/min
Monitor scan rate: about 2500nm/min
Wavelength setting
At 1nm units for scan start and scan end wavelengths, and 0.1nm units for other wavelengths
Lamp interchange wavelength
Auto switching synchronized with wavelength, switching range selectable between 282 ~ 393nm (0.1nm units)
Spectral bandwidth
6-step switching among 0.1/0.2/0.5/1/2/5nm
Response
Optimum response speed automatically set depending on bandwidth, minimum 0.1sec
Resolution
0.1nm
Stray light
UV-2450
UV-2550
Less than 0.015%
Less than 0.0003%
(220nm, NaI 10g/L solution)
Less than 0.015%
Less than 0.0003%
(340nm, UV-39 filter)
Photometric system
Double-beam, direct ratio system with dynode feedback
Photometric modes
Absorbance (Abs.), transmittance (%), reflectance (%), energy (E)
Photometric range Absorbance: -4 ~ 5 Abs
Transmittance, reflectance: 0.0 ~ 999.9%
Recording range Absorbance: -9.999 ~ 9.999 Abs
Transmittance, reflectance: -999.9 ~ 999.9%
Photometric accuracy
Ẕ0.002 Abs (0 ~ 0.5 Abs)
Ẕ0.004 Abs (0.5 ~ 1.0 Abs)
Ẕ0.3%T (0 ~ 100% T) 		} Tested with NIST 930D standard filter

* If the detector has been replaced with a near infrared sensitive photomultiplier, the standard optical specifications of the above instrument will not be satisfied.

Item
Description
Photometric repeatability
Ẕ0.001 Abs (0 ~ 0.5 Abs)
Ẕ0.002 Abs (0.5 ~ 1.0 Abs)
Ẕ0.1%T
Baseline flatness
Ẕ0.001 Abs (excluding noise, using 2nm slit, and slow wavelength scanning speed)
Baseline correction Auto correction using PC (Stored baseline is automatically loaded when power is switched on, re-correction is possible)
Drift 0.0004Abs/h (after power is on for 2 hours)
Temperature and humidity requirements 15 ~ 35°C, 45 ~ 80% (no condensation, less than 70% above 30°C)
Light source 50W halogen lamp (2,000 hours life), deuterium lamp (socket type), light source auto position adjustment built in
Monochromator UV-2450
Single monochromator, high-performance blazed holographic grating in aberration-corrected Czerny-Turner mounting
UV-2550
Grating/Grating type double monochromator, Pre-monochromator: double-blazed holographic grating Main monochromator: high-performance blazed holographic grating in aberration-corrected Czerny-Turner mounting
Detector Photomultiplier R-928
Sample compartment Internal dimensions: 150W x 260D x 120H (mm)
Distance between light beams: 100mm
Maximum light path length of cell: 100 mm
Power requirements AC100, 120, 220, 240 V, switch selectable
50/60Hz; 250 VA
Dimensions 570W x 660D x 275H (mm)
Weight About 36 kg

(PC and printer are not included.)

UVProbe Software Specifications

Operating System Windows XP Pro/2000 Pro
Instruments UV-1601 Series, UV-2401/2501 Series
Data Acquisition Modes Spectrum, Kinetics and Photometric
General
  • Multitasking (Possible to execute data processing while measurement is being executed.)
  • Customizable measurement screen layout (wavelengths, data display font and font size, colors, displayed number of rows)
  • GLP/GMP compliant (security, history)
  • Real time concentration display
Spectrum Mode
  • Comparison of multiple spectra/relative processing
  • Save all processed data with original data set including a history of all manipulations
  • Spectrum enlargement/shrinking, auto scale and Undo/Redo of these operations
  • Annotation on spectrum screen
Data Processing in Spectrum Mode
  • Normalization, Point Pick, peak/valley detection, area calculation
  • Transformations: 1st - 4th derivatives, smoothing, reciprocal, square root, natural log, logarithm power, Abs. to %T conversion, and exponential, Kubelka-Munk conversion
  • Ensemble averaging, interpolation, data set and constants arithmetic (between spectra, between spectra and constants)
Photometric (Quantitation) Mode
  • Single wavelength, multi wavelength (includes 1, 2 or 3 wavelengths), spectrum quantitation (peak, maximum, minimum, area, etc. for specified wavelength ranges)
  • Multi-point, single point, K-factor calibration curves (1st, 2nd, 3rd order function fits, pass-through-zero specification)
  • Photometric processing with user-defined functions (+, -, x,
    , Log, Exp, etc. functions, including factors)
  • Weight correction, dilution factor correction, and other corrections using factors
  • Averaging of repeat measurement data
  • Simultaneous display of standard table, unknown table and calibration curves
  • Display of Pass/Fail indications
Kinetics (Time Course) Mode
  • Comparison/relative data processing of multiple time course data
  • Single or double wavelength measurement (difference or ratio)
  • Simultaneous display of time course data, enzyme table and graphs
  • Enzyme kinetics calculation (for single or multicell)
  • Michaelis-Menten calculations and graph creation (Michaelis-Menten, Lineweaver-Burk, Hanes, Woolf, Eadie-Hofstee), Dixon plot, Hill plot
  • Unitary management of sample information including original data, sample weight and dilution factors, etc.
  • Event recording such as addition of reagents during measurement
  • Time course spectrum data processing (same as in spectrum data processing)
Report Generator
  • Preview and print functions for customized formats
  • Layout and editing of templates
  • Quick printing using report templates
  • Multi-page printout support
  • Insert date, time, text, and drawing objects including lines, circles and rectangles
  • Insert spectrum and quantitation data, method and history
  • Headers and footers easily inserted
  • Specify graph line thickness (as in all modulules), font style and size

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