February 19, 2014 | News & Notices Our paper of monoclonal antibody analysis by MS selected as Hot Articles
Our paper, Noriko Iwamoto et al., Life Science Research Center, SHIMADZU Corporation, has been selected to the Hot Articles of Analyst in Royal Society of Chemistry.
This report is one of the collaboration results of National Cancer Center in Japan.
Our new method, named nSMOL™ proteolysis (nano-surface and molecular orientation limited proteolysis), is the innovative proposal to the antibody analysis by mass spectrometry focused on the size of antibody and its orientation. Immobilized antibody on the resin with 100 nm pore has been digested by immobilized protease on 200 nm nano particles, and detected and quantified by liquid chromatography/mass spectrometry (LC/MS). This experimental design successfully performed limited proteolysis on complementarity-determining region of the antibody variable region by limiting protease access to the substrate. This nSMOL™ method is made possible therapeutic drug monitoring (TDM) independent of a variety of monoclonal antibodies, and accelerated the development of TDM system with MS.
Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis
Noriko Iwamoto, Takashi Shimada,* Yukari Umino, Chikage Aoki, Yutaka Aoki, Taka-Aki Sato, Akinobu Hamada and Hitoshi Nakagama
Analyst, 2014, 139, 576-580
This paper is available from the following link for several weeks.