Comprehensive Chromatography Guide

Comprehensive Chromatography

1-Dimensional GC/LC means usual GC, GCMS, LC, LCMS.
Comprehensive chromatography is the last destination reached by separation science. It falls into the category of 2-Dimensional GC/LC, mainly due to the association of two different mechanisms of separation. Multidimensional chromatography, where the most common method utilized, “heart-cutting” [1-4], transfers only selected portions of eluate from the first to the second dimension.
On the other hand, Comprehensive chromatography is more“comprehensive”toward the separation process involving sample analytes; in other words, this means that every portion of the eluate coming from the primary (1D) column undergoes a further 2D separation.

Comprehensive two-dimensional gas, liquid and liquid-gas chromatography hardware and software have been developed and are now exploited by a great number of analysts across the world.
GCxGC methods are making greater inroads in the analysis of fatty acid methyl esters in food and biological samples, of pesticides and petrochemicals, as well as flavours and fragrances.
LCxLC, linked with mass spectroscopy, is increasingly useful for a range of tests related to health, biology and nutrition, including proteomics, lipidomics, and food antioxidant analysis,
LCxGC methods have a demonstrated effectiveness for food contaminant analysis (e.g.,mineral oil in vegetable oils).

What is Chomprehensive Chromatography?

The unseparated compounds with the monodimensional separation system can be separated with the two-dimensional system. For example, in the same analysis time, comprehensive chromatography shows more than 200 peaks versus only 74 or 54peaks in the conventional 1D-LC system.

Separation of Overlapping Peaks

The more complex the sample, along with a high percentage of similar compounds, means there is a high probability of co-eluting compounds found under single peaks. Even though theco-eluting compounds may be unable to be separated by mono-dimensional separation, the orthogonal two-dimensional system will provide the best possible result.

Comprehensive chromatography will be contains enormous peaks. To certainly detect these peaks, UFMS (Ultra Fast Mass Spectrometry) must be need. Shimadzu's UFMS technologies, which minimize sensitivity losses even at faster scan speeds, can detect these peaks.

Reference

  • [1] Deans, D.R.; Huckle, M.T.; Peterson, R.M. Chromatographia, 1971, 4, 279-285.
  • [2] Dugo, G.; Dugo, P.; Mondello, L. Multidimensional Chromatography; Mondello, Lewis and Bartle, Eds.; John Wiley & sons, England, 2002; pp. 217-250.
  • [3] Jennings, W. Analytical Gas Chromatography, Academic Press: Orlando, FL, USA, 1987.
  • [4] Mondello, L.; Catalfamo, M.; Proteggente, A.R.; Bonaccorsi,I.; Dugo, G. J. Agric. Food Chem.,1998, 46, 54-61.

 

For Research Use Only. Not for use in diagnostic procedures.

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