The system includes HPLC (Prominence) units, LC-GC transfer unit (AOC-5000 Plus and OPTIC-4PTV), GCxGC unit (GC-2010 Plus and ZX-2), and a MS/MS (GCMS-TQ8040). After the sample is injected into the Prominence HPLC, it is separated by normal-phase chromatography. Then the AOC-5000 Plus injects only the target fraction into the OPTIC-4 GC inlet. After separating the fraction in the GCxGC with a double-oven and ZX-2 thermal modulator, it is detected in the GCMS-TQ8040. This entire series of analytical steps can be executed entirely from the 5D Solution integrated software, which means the resulting data can be analyzed using ChromSquare 2D chromatography data analysis software.
*1: The OPTIC-4PTV is a product of GL Sciences B.V.
*2: The ZX-2 is a product of Zoex Corporation in the United States.
Dedicated software is used to control each unit of the LC-GCxGC-MS/MS system. However, 5D Solution collectively manages all of these control software programs, so that analysis can be started with a single click. Furthermore, continuous analysis of multiple samples can be performed.
The included linear velocity calculation software allows the user to view the calculated linear velocity values of the first and second columns while the optimal column inlet pressures are being specified, which provides powerful assistance for determining analytical conditions for comprehensive 2D-GC analysis.