Large Scale Preparative System
Preparative Chromatography Systems
Large Scale Preparative System
Using the Same System to Evaluate Fractionation Parameters and for Large-Scale Fractionation
With a maximum flowrate of 150 mL/min, this Prominence preparative liquid chromatograph is capable of automatic continuous fractionation using preparative columns with internal diameters up to 50 mm.
Equipped with a new LC-20AP preparative pump capable of high core performance levels at a wide range of flowrates, ranging from levels for analysis to large scale fractionation, this single system can handle all work flows desirable for preparative liquid chromatographs, from evaluating fractionation conditions to confirming purity.
1.High Speed and High Purity Fractionation
Using columns with high separation capability enables high speed fractionation.
To collect component fractions from samples containing complex mixtures, it is desirable to use a high-separation column packed with small particles. However, increasing the flowrate through such a column increases the pressure. Therefore, the new LC-20AP preparative pump features improved pressure resistance at high flowrates (42 MPa for flowrates up to 100 mL/min or 30 MPa even for flowrates up to 150 mL/min). This enables using high-separation or high-performance columns, which means high-purity fractionation can be expected, even when target compounds are contained in trace amounts, such as impurities in pharmaceuticals.
2.Enables Building Systems Capable of Both Analysis and Fractionation
In addition to improving flowrate accuracy to ±1 % or less, which is two times better than before, even at analytical flowrates (about 1 mL/min) used for evaluating fractionation parameters, this also reduces the pulse flow during solvent delivery and provides the same delivery performance level as analytical solvent delivery units. This means the same system can be used not only for large scale preparative purification, but also for evaluating fractionation parameters with high reliability and for confirming the purity of fractions and purified compounds.
3.Efficient Fractionation Using MS Triggers
Fractionation using UV chromatograms results in fraction profiles that depend on retention times and peak integration. Therefore, fraction spectra can include peaks from components other than the target compounds, which could prevent efficient fractionation.
By combining the system with an LCMS system, structural information about the compounds purified by fractionation can be obtained at the same time as fractionation. Since fractionation is based on the masses specified for a target compound, reliable fractions can be obtained using simple fractionation parameter settings. Furthermore, this allows setting the analysis time for confirming the target compound to zero, which used to be performed after fractionation.
4.Energy Savings Achieved, with 40 % Lower Power Consumption Than Previous Model
The new LC-20AP preparative pump achieves a 40 %* reduction in power consumption, compared to Shimadzu's previous model. This contributes to saving energy. Furthermore, the system is about 25 % more space efficient than the LC-8A (previous model), with the width reduced from 350 mm to 260 mm.
* Comparison of measured values for LC-20AP and LC-8A models, given a pressure of 5 MPa and flowrate of 40 mL/min.
5.Improved System Availability Rate
In combination with the Web-based software Open Solution, fractionation can be accomplished easily in 3 steps - log in, register samples, and execute. No complicated operator training is necessary. Fractionation results can be quickly confirmed via a local area network with a standard Web browser, even from a separate room. By providing an open access type environment like this, the availability rate of preparative liquid chromatographs, which are often used as shared equipment, can be improved dramatically.
Fractionation of Paeoniflorin in Radix Paeoniae
Column: Shim-pack PREP-ODS
(250 mmL × 50 mm I.D., 15 µm)
Mobile Phase: Acetonitrile / water = 6/1
Flowrate: 100 mL/min
Sample: 200 mL peony powder extract solution (equivalent to 2 g of powder) injected by pump
Purification of Glycyrrhizinate for Food Additive Testing
Column: Shim-pack PREP-ODS (250 mmL × 50 mm I.D., 15 µm) Mobile Phase: 2 % (v/v) aqueous acetic acid/acetonitrile = 65/35 Flowrate: 70 mL/min Sample: 1 g/100 mL ammonium glycyrrhizinate for food additive testing injected by pump