SEC(GPC)-MALDI TOF MS
Trace Analysis of By-Products and Additives in Synthetic Polymers
MALDI-MS is widely used as an analytical tool for the characterization of synthetic polymer samples. However, in complex mixtures, the minor components may not be detected due to ion suppression by more abundant species also present in the sample. Gel Permeation Chromatography (GPC)-MALDI is an effective method for detailed analysis of such samples. In GPC-MALDI, complex samples are fractionated by GPC prior to MALDI-MS analysis. When preparing polymer samples for MALDI-MS analysis, the sample (e.g., an aliquot of a GPC fraction) must be mixed with MALDI matrix and a salt (to promote cationization of the analyte) prior to loading on to the MALDI target. To automate this process, we have developed an organic solvent-resistant fraction collector / MALDI spotting robot (AccuSpot), which mixes the GPC eluent with MALDI matrix solution on-line and then automatically deposits the resulting solution onto the MALDI sample target. The new AccuSpot provides a fully automated solution for the GPC-MALDI analysis of synthetic polymer samples.
Detection and Structural Analysis of Trace Amount of Samples
- In addition to the measurement of molecular weight distribution of SEC (GPC) system with refractive index detector, MALDI-TOFMS provides detailed structural information such as structure of monomers in polymers.
- Mass spectrometry after separation by SEC decreases the ionization suppression by main component, and makes easier to detect and perform structural analysis of trace amount of samples.