Shim-pack GIS Series

HPLC Column Solutions

High retentivity, lower column back pressure, high inertness

Analysis Examples

Food Dyes

Peaks

 1. Tartrazine  7.6 mg/L
 2. Amaranth  3.8 mg/L
 3. Ingigocarmine  7.6 mg/L
 4. New coccine  3.8 mg/L
 5. Sunset Yellow FCF  5.3 mg/L
 6. Naphthol Yellow S  7.6 mg/L
 7. Uranine  3.8 mg/L
 8. Allura Red AC  5.3 mg/L
 9. Ponceau R  7.6 mg/L
 10. Ponceau SX  5.3 mg/L
 11. Orange I  5.3 mg/L
 12. Fast green FCF  3.0 mg/L
 13. Brilliant blue FCF  3.0 mg/L
 14. Ponceau 3R  7.6 mg/L
 15. Erythrosine  5.3 mg/L
 16. Azure Blue VX  3.0 mg/L
 17. Orange II  7.6 mg/L
 18. Acid red  3.0 mg/L

 

Conditions

 Column  Shim-pack GIS C18 (150 mmL. x 4.6 mmI.D., 4 μm)
 Eluent  A) 10mM Na2HPO4 (pH6.9 ; 10mM NaH2PO4)
 B) CH3CN
 A/B = 90/10 - 50 min - 65/35
 Flow Rate  1.0 mL/min
 Detection  UV 270 nm
 Col. Temp.  40 °C
 Injection Vol.  10 μL

Free Amino acids and Organic acids

Conditions

 Column  Shim-pack GIS CN
 (250 mmL. x 4.6 mmI.D., 5 μm)
 Eluent  20 mM KH2PO4 (pH 4.0; H3PO4)
 Flow Rate  1.0 mL/min
 Col. Temp.  40 °C
 Detection  UV 210 nm
 Injection Vol.  5 μL

Specifications

  Shim-pack
GIS C18
Shim-pack
GIS C18-P
Shim-pack
GIS RP-Shield
Shim-pack
GIS CN
Shim-pack
GIS HILIC
Bonded Phase Octadecyl Groups Octadecyl Groups Octadecyl Groups Cyanopropryl Group Diol Group
Analysis Mode Reversed Phase Reversed Phase Reversed Phase Reversed Phase, Normal Phase HILIC
Features High retentivity, lower columnback pressure, high inertness High Steric Selectivity Embedded with a Polar Functional Group Suitable in either reversedphase or normal phase mode Ideal for the Separation of Highly Polar Basic Compounds
Particle Size (μm) 2, 3, 4, 5, 10 3, 5 5 3, 5 3, 5
Pore Size (nm) 10 10 10 10 10
Surface Area (m2/g)
450 450 450 450 450
Carbon Loading (%) 15 29 9 14 20
End Cap - - - -
pH Range 2-7.5 2-7.5 2-7.5 2-7.5 2-7.5
USP Code L1 L1 L1 L10 L20

For Research Use Only. Not for use in diagnostic procedures.

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