Co-Sense for LC-NMR

Co-Sense for LC-NMR

In LC-NMR, large amounts of expensive deuterated solvent are required to eliminate interfering signals originating from the HPLC solvent. Further, inadequate sensitivity due to data sampling cannot be compensated for online, precluding the applicability of NMR alone for trace analysis. Therefore, when analytes consist of trace levels of impurities in pharmaceutical products, complicated HPLC elution processes are employed to collect the analytes for subsequent analysis by NMR. Co-Sense for NMR automates the entire process of separating and purifying such target compounds from the sample, including the processes of concentration, desalting and deuterated solvent replacement. This effectively shortens the LC-NMR measurement time and decreases overall operation costs.


Fully Automated Sample Processing

All sample processing, from introduction, separation and concentration of target compounds, desalting and solvent replacement, to transfer to the NMR sample tube, has been completely automated. This enhances operation efficiency, and eliminates errors associated with performing manual tasks. Online continuous processing also allows reliable, repeatable analysis even with unstable samples.

Higher Sensitivity Measurement

Concentrations of target compounds can be increased several x ten-fold compared with existing LC-NMR, enabling acquisition of high S/N NMR spectra in a shorter time for higher throughput.

Optimization of HPLC Parameters

HPLC separation parameters can be set without regard for subsequent NMR measurement, relieving any constraints in setting the optimum separation parameters.

Easier Operation

The system is controlled via graphical user interface software. With easy operation, the system operation status is known at a glance.

Reduced Solvent Usage

In the Co-Sense LC-NMR, during the large volume solvent consumption phase of fraction collection, only typical HPLC solvents are used. Since only extremely small amounts of high-cost deuterated solvents are used to elute target compounds from the trap column, operation costs are reduced.

1. Separation: Separation of target compounds is performed using a preparative column, and the eluat

1. Separation: Separation of target compounds is performed using a preparative column, and the eluate is accumulated in a sample loop.


2. Concentration: The eluate inside the sample loop is introduced into a trap column for adsorption, desalting and concentrating.


3. Substitution: Heavy water (D2O) is delivered to replace the solution in the trap column.


4. Purification: Deuterated solvent is delivered to elute the target compound from the trap column, and after further purification, the eluate is sent directly to the NMR tube.

For Research Use Only. Not for use in diagnostic procedures.

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