i-Series Plus

LC Systems

Analysis of Sugars in Juice

The new i-Series Plus integrated high-performance liquid chromatograph can be connected to the RID-10A differential refractive index detector. Since the column oven can accommodate a 30-cm column for use in sugar analysis (ligand exchange column), and the temperature can be controlled up to 85 °C, it therefore supports applications that require a long column and high column temperature.
Here, we introduce an example of sugar analysis in juices using the Prominence-i with the RID-10A.

Analysis of Sugar Standard Solution

Fig. 1 shows the results of analysis of a standard mixture of four sugars (maltotriose, sucrose, glucose, fructose) using a 10 μL injection (each at 20 g/L). For the analytical column, we used the Shim-pack SCR-101N, a specialized sugar-analysis column that supports both the gel filtration and ligand exchange modes.

Analytical Conditions
Column : Shim-pack SCR-101N (300 mm L. x 7.9 mm I.D., 10 μm)
Mobile Phase : Water
Flowrate : 0.6 mL/min
Column temp. : 80 °C
Injection Volume : 10 μL
Detection : RID-10A Polarity +, Cell temp. 40 °C, Response 1.5 sec

Analysis of Orange Juice

Fig. 2 and 3 show the chromatograms obtained from analysis of Orange Juice A and Orange Juice B, respectively. Both Orange Juice A and B were diluted with water to obtain 10-fold dilutions, respectively, and after filtering the solutions through a 0.2 μm membrane filter, 10 μL each was injected. Sucrose, glucose and fructose were detected in both types of orange juice.


For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

Top of This Page