Nexera Method Scouting System

Ultra High Performance Liquid Chromatograph Method Scouting System

Rapid Method Scouting of Chiral Compounds

Rapid Method Scouting of Chiral Compounds

Typically, investigations of analysis conditions for chiral compounds require a lengthy trial-and-error process in which multiple column and mobile phase combinations are examined in order to determine the appropriate one for separation of the target analyte. Quicker, more efficient investigations can be conducted by combining Shimadzu's Nexera Method Scouting, used as the screening system for the analysis conditions, with the Daicel iCHIRAL-6 series solvent-resistant chiral columns, used as the optical resolution columns.

1. Using Nexera Method Scouting for Investigating Chiral Analysis Conditions

 

  • This is a high-pressure resistant, robust column switching system. It was designed based on the Nexera X2, an all-round LC system that can be used for a wide range of applications from conventional to UHPLC analysis.
  • It automatically switches between six columns, eight mobile phases, and their mixing ratios to achieve a rapid and accurate method scouting sequence.
  • It can accommodate analysis conditions for either normal-phase chiral analysis or reversed-phase chiral analysis.

 

2. Features of theiCHIRAL-6 Series Solvent-Resistant Chiral Columns

In Daicel solvent-resistant chiral columns, the polysaccharide derivatives, which serve as the chiral selector, are immobilized on silica gel. In addition to alkane and alcohol, all solvents used with HPLC, including ethyl acetate, THF, and halogen-based solvents, can be used as the mobile phase and the sample solvent.

There are a total of six columns in theiCHIRAL-6 series solvent-resistant chiral columns: CHIRALPAKâ IA, IB, IC, ID, IE, and IF*. They are complementary for separation targets, so a wide range of compounds can be optically resolved.

In Daicel solvent-resistant chiral columns, the polysaccharide derivatives, which serve as the chiral selector, are immobilized on silica gel. In addition to alkane and alcohol, all solvents used with HPLC, including ethyl acetate, THF, and halogen-based solvents, can be used as the mobile phase and the sample solvent.

*CHIRALPAKâ is a registered trademark of Daicel Corporation.

3. Combining Nexera Method Scouting with theiCHIRAL-6 Series

This section shows the results of screening a total of 48 normal phase analysis conditions, with the combinations of the DaiceliCHIRAL-6 series and eight mobile phase conditions indicated at right, using Nexera Method Scouting.

Nexera Method Scouting enables analysis to be performed while switching the mobile phases and columns automatically. As a result, analysis can be performed consecutively day or night, enabling rapid investigation of conditions. In the example at right, the data sampling was completed overnight. All that the analyst has to do to collect the data is to set up the mobile phases and samples, and start the analysis, which results in significant labor savings. In addition, while the analysis is being performed, the analyst can engage in other tasks, thereby improving work efficiency.

Scouting Conditions

In the analysis process after data collection, the data browser function in LabSolutions, Shimadzu's HPLC workstation, can be used to tabulate large amounts of scouting data. It displays the data together with indices for chromatogram evaluations, such as retention times, area values, resolution, and tailing factors. This allows for rapid, accurate analysis of the scouting data for chiral resolution conditions.

Scouting Results

For Research Use Only. Not for use in diagnostic procedures.

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