Preparative system general HPLC: Nexera Prep

Components Difficult to Separate can be Recovered at High Purity Levels and at Low Cost

What is the recycling separation method?

Long preparative columns are expensive, as such there is a need to use comparatively lower cost short columns effectively.
In the recycling separation method (closed valve recycling), the eluate liquid containing the target components that has eluted from the separation column is recycled into the column, enabling an equivalent separation capacity to that of a longer column.

Flow Lines for the Recycling Preparative System


The figure below shows the results of a seven-cycle recycling separation. In the first injection (typical separation), the separation of the two components is insufficient (red area). However, when the column eluate is returned to the column from the detector, it is separated a second time. If this recycling is repeated, the results obtained are equivalent to connecting a number of columns in series corresponding to the number of repetitions. In this example, a 4.0 or better resolution was ultimately obtained with seven recycling separation cycles (blue area).

Example of the improvement in separation by recycling


Example of the improvement in separation by recycling : The coeluting peaks (red) are completely separated (blue).
Flowrate : 10 mL/min
Detection wavelength : 254 nm
Column : Shim-pack PREP-ODS(H) 20 mm I.D. x 250 mm L.
Mobile phase : Water/methanol = 1/9 (v/v)
Sample : Mixed 1% n-butylbenzene/iso-butylbenzene solution


Recycle-Assist - Special Preparative Recycling Software*

Perform Automatic Preparative Recycling with a Simple GUI-Based Operating Environment

The graphical user interface (GUI) provides an environment where even novices to preparative recycling can perform operations simply and reliably. A single main window is used for the workflow from recycling to fractionation, thus reducing the risk of wasting precious samples through setting mistakes.

Recycling Conditions are Completely Set in Three Steps

Just click three points corresponding to the recycling start, recycling stop, and automatic collection start point to complete the settings for the recycling preparation conditions. Entering complicated numerical parameters is completely unnecessary, so recycling preparative work can start easily.

* This is compatible with the FRC-10A

For Research Use Only. Not for use in diagnostic procedures.

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