Preparative system general HPLC: Nexera Prep

System Configuration Applicable to a Variety of Applications

Solvent Delivery Unit Accommodates a Wide Range of Recovery Volumes

Guidelines for Preparative Scale and Maximum Load

Guidelines for Preparative Scale and Maximum Load

The figure at right shows the guidelines for total component capacity with a 250 mm long column when the target component is highly soluble in the mobile phase, separates from impurities, and ions are suppressed. For isocratic elution, in principle, the total component capacity is proportional to the column volume.

 

Guidelines for Preparative Scale and Maximum Load

LC-20AT

  • This solvent delivery unit can handle flow rates ranging from those used in analytical scale to those used in semi-preparative (up to 10 mL/min).
  • High-precision analysis is possible even in the semi-micro flow-rate range.
 

Supports Semi-Preparative and Recycle Preparative

LC-20AR

  • This solvent delivery unit can handle flow rates used in semi-preparative scale (up to 20 mL/min).
  • Using a recycle kit enables semi-preparative recycling.
 

Supports Large-Scale Preparative Fractionation

LC-20AP

  • High flow rates (up to 150 mL/min) enable highly efficient, large-scale preparative fractionation.
  • Large-scale prep solvent delivery fully supports the preparative fractionation workflow including reinjection to assess purity.
  • Combine with an FCV-200AL low-pressure gradient unit to perform gradient analysis using up to four mobile phases.
 
  LC-20AT LC-20AR LC-20AP
Solvent delivery method Series-type double plunger Parallel-type double plunger
Plunger capacity Primary side: 47 μL, Secondary side: 23 μL 47 μL 250 μL
Maximum discharge pressure 40 MPa 49 MPa 42 MPa
Flow rate setting range 0.001 to 10.000 mL/min 0.001 to 20.000 mL/min 0.01 to 150.00 mL/min
Flow rate accuracy
No more than ±2% or ±2 μL/min,
whichever is greater (0.01 to 5 mL/min)
No more than ±1% or ±10 μL/min,
whichever is greater (0.1 to 5.0 mL/min)
No more than ±1% (1 mL/min, 10 MPa)
Flow rate precision No more than 0.06% RSD or 0.02 min SD,
whichever is greate
No more than 0.08% RSD or 0.02 min SD, whichever is greater No more than 0.1% RSD or 0.02 min SD,
whichever is greater
Constant pressure solvent delivery Supported
Plunger rinsing mechanism Syringe or rinsing pump Syringe or rinsing pump
Operating temperature range 4 to 35 °C 4 to 35 °C 4 to 35 °C
Size and weight W 260 x H140 x D 420 mm,11 kg W 260 x H140 x D 500 mm,16 kg W 260 x H210 x D 500 mm,19 kg

Shim-pack Scepter Columns

- Excellent stability & performance using a wide range of LC conditions -

Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns, are designed for stability and performance in a wide range of mobile phase conditions. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting under conditions that may compromise traditional silica based columns.
With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC, making method transfer seamless between different laboratory instrumentation.

Shim-pack Scepter Reversed Phase
C18 HD-C18 C8 Phenyl PFPP
Functional Group Trifunctional C18 Trifunctional C18 Trifunctional C8 Trifunctional
Phenylbutyl
Trifunctional
Pentafluorophenylpropyl
Generic Purpose Type High Density Type
Particle Organic Silica Hybrid
Particle Size 1.9 μm,3 μm,5 μm
Pore Size 12 nm 8 nm 12 nm
End Capping Proprietary None
pH Range 1 - 12 1 - 10 1 - 8
100% Aqueous Condition Yes No No Yes Yes
USP Classification L1 L1 L7 L11 L43

The LH-40 Liquid Handler, Combination of Autosampler and Fraction Collector

LH-40 Liquid Handler

Options

Syringe Kit 20 mL

This kit enables large-capacity injections of 2 mL or more at one time. The maximum injection volume is 20 mL.

Washing Pump

This reduces the washing time for the injection needle, increasing throughput while reducing carryover.

 

Analysis Kit

The recovered fraction can be reanalyzed to check the purity.

Liquid Surface Detection Needle

This detects the liquid surface level, and automatically determines whether there is any sample present. As a result, only the remaining volume is injected, which prevents the injection of air into columns. Additionally, if no sample is present, the system can proceed to the next sample, reducing needless errors and lost labor.

 

 

Autosampler

SIL-10AP

Sample Racks
・Sample rack S for 1.5 mL vials
・Sample rack L for 4.0 mL vials
・Sample rack LL for 13 mL vials*1
*1 Sample rack LL is a standard accessory of the SIL-10AP

Sample Coolers

Block Cooling/Heating: 4 to 70 °C)

Sample Coolers

Manual Injecto

Rheodyne®7725

Optional Sample Loops (Material: SUS)

Manual Injector

Volume Part Number(P/N)
100 μL 228-32211-16
200 μL 228-32211-17
500 μL 228-32211-18
1 mL 228-32211-19
5 mL 228-32211-21

Specifications

  LH-40 SIL-10AP
Maximum Number of Containers Up to 540 pcs* (10 mm I.D. test tubes) Up to 100 pcs* (1.5 mL vials)
Maximum Injection Volume Up to 20 mL (with the Large Capacity Injection Kit) Up to 5 mL
Cooling Function None Yes (Optionally available)
Compatible Containers and Quantities
10 mmO.D. test tube 540 pcs
12 mmO.D. test tube 486 pcs
16 mmO.D. test tube 216 pcs
18 mmO.D. test tube 216 pcs
25 mmO.D. test tube 108 pcs
35 mmO.D. test tube 54 pcs
MTP/DWP 9 pcs
1.5 mL vials 100 pcs
4.0 mL vials 80 pcs
13 mL vials 25 pcs
Compatible Containers and Quantities W 390 x H 560 x D 730 mm, 40 kg W 260 x H 280 x D 320 mm, 18.5 kg

* Depends on the type of racks used

 
 
 

FRC-40, Highly Flexible Fraction Collector

Fraction Collector

Sample Racks


A variety of containers can be placed including MTPs, vials, and various types of test tubes. Six colors are available, so a separate color can be apportioned to each user in order to avoid confusing samples.

Sample Racks

Multi Fraction Collector Kit

Up to six FRC-40 fraction collectors (up to five LH-40 liquid handlers) can be connected, making it easy to increase the number of fractions.

FRC-10A

Compact Design for Small-Volume Samples

FRC-10A

For smaller scale collection, or specialized applications that require enclosure and cooling, the FRC-10A is a compact fraction collector that provides time and signal based triggering. A variety of programmable fractionation functions enable target components to be collected with high purity and high recovery.。

Specifications

  FRC-40 FRC-10A
Maximum Number of Fractions Up to 540 pcs* Up to 144 pcs*
Maximum Flow Rate 150 mL/min
Fractionation Mode Configured through a combination of basic mode (initial parameter mode) and time program mode (14 parameters)
Cooling Function None Optionally available
Compatible Containers and
Quantities
10 mmO.D. test tube   540 pcs 4 mL vial 252 pcs
12 mmO.D. test tube 486 pcs 15 mL vial 108 pcs
16 mmO.D. test tube 216 pcs 50 mL vial 54 pcs
18 mmO.D. test tube 216 pcs 250 mL vial 20 pcs
25 mmO.D. test tube 108 pcs 500/1000 mL bottle 12 pcs
35 mmO.D. test tube 54 pcs MTP/DWP 9 pcs
10 mmO.D. test tubes 144 pcs
18 mmO.D. test tubes 164 pcs
35 mmO.D. test tubes 16 pcs
Size and weight W390 × H560 × D730 mm, 30 kg W 260 x H 280 x D 420 mm,15 kg
* Depends on the type of racks used.
 
 
 

Example of Scaling Up from Analytical to Semi Preparative Work

Example of Scaling Up for Parabens

This is an example of scaling up in which seven types of parabens are targeted using a 150 mm long column with a particle size of 5 μm.
The gradient elution conditions investigated at the analytical scale are transitioned to the semi-preparative scale. A comparable chromatogram is obtained at both scales.

Column: Shim-pack Scepter C18-120 (4.6 mm x 150 mm,5 μm)
Column: Shim-pack Scepter C18-120 (20 mm x 150 mm,5 μm)

 

For Research Use Only. Not for use in diagnostic procedures.

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