Nexera UC Prep

Extensive column lineup opens up analysis options

Shim-pack UC series columns are designed specifically for Nexera UC series SFC systems. When using supercritical fluids for analysis, separation behavior can vary significantly depending on the type of solid phase. To optimize separation, a variety of columns should be used to determine the parameter settings. The extensive choice of column sizes available means that operations can be scaled up seamlessly from analytical SFC to preparative SFC.

  Functional group 4.6×250mm 10×250mm 20×250mm 28×250mm
Shim‐pack UC-Diol Ⅱ Diol
227-32606-03 227-32606-04 227-32606-05
Shim‐pack UC-Sil Ⅱ 227-32607-02 227-32607-03 227-32607-04 227-32607-05
Shim‐pack UC-HyP 3-hydroxphenyl 227-32600-02 227-32600-03 227-32600-04 227-32600-05
Shim‐pack UC-Py Pyridinyl 227-32601-02 227-32601-03 227-32601-04 227-32601-05
Shim‐pack UC-PBr Pentabromobenzyl 227-32602-02 227-32602-03 227-32602-04 227-32602-05
Shim‐pack UC-Choles Cholesteryl 227-32603-02 227-32603-03 227-32603-04 227-32603-05
Shim‐pack UC-PyE Pyrenylethyl 227-32604-02 227-32604-03 227-32604-04 227-32603-05
Shim‐pack UC-Triazole Triazole 227-32605-02 227-32605-03 227-32605-04 227-32605-05

Choosing a column

Since normal phase separation is the main separation mode used for SFC, normal phase UC-Diol II columns are commonly used. UC-Diol II columns can be used for analyzing a wide variety of compounds, from phospholipids to highly-polar peptides. UC-Py columns exhibit similar behavior to ethylpyridine-based columns, and also display very versatile performance.

In addition, UC-HyP columns can uniquely separate lipids by class.
Columns with multiple interaction modes can improve separation of isomers or compounds that are difficult to separate by LC. Prime candidates include the UC-Choles, which contains a cholesteryl group, the UC-PyE, with strong π-π interactions, and the UC-PBr, which applies a dispersive force to Br.

Vitamin D isomer

Successive analysis without changing the mobile phase

In HPLC analysis, water-based mobile phases are generally used for reverse phase analysis, and non-water based mobile phases for normal phase analysis. However, in SFC analysis a mixture of supercritical carbon dioxide and a modifier (an organic solvent such as methanol) is used as the mobile phase, regardless of the solid phase. This means the same mobile phase composition can be used for successive analysis with all columns.

Method scouting for optimization of separation conditions and scaling up to preparative size

Method Scouting Solution
Method Scouting Solution

For high-purity preparative analysis, target peaks e.g. of different chiral compounds need to be adequately separated, for which the user must determine optimal column and separation parameter settings. The Nexera UC chiral screening system with dedicated Method Scouting Solution software can be used to screen columns more quickly and accurately (Step 1) and smoothly scale up to preparative scale flowrates (Step 2).

Method scouting at analysis scale

Method scouting is easy even for first-time users – simply execute the batch table generated automatically by the dedicated software. The system can automatically switch between different settings to run the scouting process continuously day or night, even for multiple modifiers and columns. Various types of data can be displayed in the data browser and multi-data reports generated with separation values for all data to assist in evaluating separation conditions.

Using a Shim-pack UC series column enables you to increase loading rates while maintaining separation performance. The optimal preparative column determined from Step 1 can be used to scale up the column size, flow speed, and injection volume based on the desired fraction volumes.

Shim-pack UC series column

Shim-pack UC series 

For Research Use Only. Not for use in diagnostic procedures.

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