High precision, stability and durability
Good repeatability is a strong feature of an HPLC. This feature must not be sacrificed by high speed.
Prominence UFLC employs a proven micro-stroke solvent delivery mechanism (flow rate resolution 3.7nL/min., 10uL/stroke) with improved firmware so that ultra-fast gradient formation occurs. The Prominence Autosampler, with its highly sealed measuring device and high-precision valves, achieves excellent repeatability for fast LC.
Repeatability in small-volume injection and ultra-fast analysis
In general high-speed LC, repeatability in a small sample volume is often regarded as a problem. The figure below shows a chromatogram by Prominence UFLC for a 2uL injection and one-minute gradient analysis. Repeatability of less than 0.2% RSD is achieved.
Column: Shim-pack XR-ODS (50mmL. x 3mm i.d.), Temperature: 40°C. Mobile Phase: Water/Acetonitrile gradient elution, Flow Rate: 1.5mL/min, Detection: 245nm, Sample: Alkylphenones, Injection Volume: 2uL
|Peaks||Retention time||Peak area|
For precise equipment control, required by analytical validation processes and quality control tests, and for minimizing the risk of a system malfunction interrupting an analysis, the most important factor is the high-pressure valve on the autosampler. The Prominence UFLC uses Shimadzu’s own proprietary high-precision 6-port valve, which achieves 0.1% or less (RSD) area repeatability in 100,000 cycle endurance tests*.
In addition, for LC-MS analyses where reduction of sample carryover has currently gained a great deal of attention, Prominence UFLC successfully reduces carryover based on the principle of inhibiting the adsorption of samples in the first place.
* Based on Shimadzu endurance test parameters for 6-port valve.
Repeatability for 4 Alkyl Phenons After Finishing 100,000 Cycle Endurance Test