Q: How fast should I set the mobile phase flow rate when using a high-speed analysis column?

Use of a semi-micro column, Higher speed

Q: How fast should I set the mobile phase flow rate when using a high-speed analysis column?

A:

In general, consideration of the Van Deemter equation indicates that using packing consisting of smaller particles improves both the column efficiency and the optimum mobile phase line speed. For the Shim-pack XR-ODS too, with its particle diameter of 2.2 µm, the appropriate mobile phase flow rate is higher than it is for a column with a particle diameter of 5 µm. The table indicates the optimum mobile phase flow rate ranges for all the different inner diameters of Shim-pack XR-ODS columns and so set the mobile phase flow rate after referring to this table and considering the pressure resistance of the system and column.


For Research Use Only. Not for use in diagnostic procedures.

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