Q: If acetonitrile is replaced with methanol, the pressure applied to the column increases. Is there any influence on the UV detector cell?

Switching the mobile phase from acetonitrile to other (methanol, etc.)

Q: If acetonitrile is replaced with methanol, the pressure applied to the column increases. Is there any influence on the UV detector cell?

A:

The column is the part in HPLC channels that is subjected to the greatest pressure. Beyond the column outlet, there is no resistance and so hardly any pressure is applied. A methanol-based mobile phase can be used with a standard detector cell without any problems.

For Research Use Only. Not for use in diagnostic procedures.

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