Sugars in Miso
A differential refractive index detector is normally used for the high-performance liquid chromatography analysis of sugars. However, in the case of fermented samples that contain many impurities, the fluorescence detector is more suitable if the sugar content is low. As sugars themselves do not fluoresce significantly, after separation in the column they are subjected to thermal reaction with arginine to form fluorescent derivatives.
The diagram shows an analysis of the sugars in miso (fermented soybean paste) using the anion exchange method and a fluorescence detector.
Reducing Sugar Analysis System
This system allows the selective, high-sensitivity detection of sugars in fermented samples that contain many impurities by post-column fluorescence detection. This is the ideal instrument for the analysis of trace quantities of sugars, such as the analysis of the sugar chains of glycoproteins.