Vitamins play important roles in the metabolic activities of the body. However, since they cannot be synthesized in sufficient quantities in the body, they must be ingested either in our meals or by other means, such as tablets. 13 kinds of components are recognized as vitamins in human, and their work varies according to their type. Vitamins can be classified into two groups, water-soluble (vitamin B group, C group etc.) and fat-soluble (vitamins A, D, E, K), and their analysis is generally conducted by high-performance liquid chromatography.
Simultaneous Analysis of Water-Soluble Vitamins (HPLC)
The water-soluble vitamins consist mainly of highly polar basic components which exhibit weak retention in analysis by reversed-phase liquid chromatography. Since they exhibit weak retention, the analysis is typically conducted using ionpair reagents. Using ion-pair reagents can be challenging because of the longer analysis time, widening of slow eluting peaks, difficulty in obtaining sufficient sensitivity and long equilibration times. Analysis efficiency is also compromised due to additional work-flow requirements, such as mobile phase preparation and column conditioning. Here, we introduce an example in which developed "Shim-pack MAqC-ODS Ⅰ" column is used to conduct simultaneous analysis of water-soluble vitamins without the addition of ion-pair reagents to the mobile phase.