The analysis of vitamins by high-performance liquid chromatography (HPLC) can be broadly categorized into the analysis of water-soluble vitamins and the analysis of fat-soluble vitamins.
The diagram shows the analysis of fat-soluble vitamins, the tocopherols, in milk using chloroform extraction.

Analysis of Tocopherols in Milk

High-Performance Liquid Chromatograph

Liquid Chromatograph Mass Spectrometer

A liquid medium flows through the flow channels of a high-performance liquid chromatograph (HPLC). When a sample is introduced into the instrument, the components of the sample are separated in a column and the amount of each component is measured by a detector
Use a system incorporating a UV detector or high-sensitivity fluorescence detector for this analysis.