Analysis of Allergens

Food allergy is becoming a major concern for public health and food industries. To avoid unexpected contact with food allergens, food labeling systems are applied to indicate presence of specific allergens in many countries.

Analysis of food allergens (LC-MS/MS)

An example of simultaneous analysis of allergens using LC-MS/MS is shown, which contains 150 MRMs for 31 peptides of 13 allergenic proteins identified from 8 foods regulated in the US (milk, eggs, fish, crustacean shellfish, tree nuts, peanuts, wheat and soybeans). They were successfully detected with high sensitivity and selectivity.

Chromatogram of peptides mixture derived from 8 food allergens,  and magnified view of 5 MRM transitions for wheat peptides and its calibration curve

Chromatogram of peptides mixture derived from 8 food allergens,  and magnified view of 5 MRM transitions for wheat peptides and its calibration curve

 
 
Chromatogram of different breads

Chromatogram of different breads

Analytical results of allergens in food samples​

Food Allergens Bread
Gluten free
bread
Peanuts
cookies
Frozen fish
"fried cod"
Frozen pasta
"garlic shrimp"
Label Detect
Label
Detect
Label
Detect
Label
Detect
Label
Detect
Milk Bos d 8
Bos d 5
        -
×
×
×
×
×
×
×
×
Egg Gal d 2
Gal d 3
× ×
×
    -
×
×
       
Atlantic cod Gad m 1             × ×    
Whiteleg shrimp Lit v 1
Lit v 3
Lit v 4
        -       ×
×
×
×
Almonds Pru du 6         -          
Peanuts Ara h 1         - ×        
Wheat Tri a 26
Tri a 36
×
×
×
    -
×
×
×
×
×
×
×
×
Soybeans Gly m TI     × × -       ×  

Detection of Allergenic Substances (Electrophoresis System)

Japan was the world's earliest adopter of a labeling system for foods containing allergens. DNA analysis by qualitative PCR can be performed on five (wheat, buckwheat, peanuts, prawn, and crab) of the seven specified raw materials (excluding egg and milk) .

Analytical results for PCR products from allergenic substances

Analytical results for PCR products
from allergenic substances

Related Information (Including Old Applications)

For Research Use Only. Not for use in diagnostic procedures.

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