Nucleotides in Fresh Tuna Meat

Reversed-phase chromatography can quantify a total of six components – four types of nucleotides, hypoxanthine and inosine; therefore, this technique can be applied to the measurement of K values (freshness index) in seafood.
The following example shows the separation of eight adenine derivative components in tuna meat by high-performance liquid chromatography (HPLC).

Analysis of Tuna Meat

High-Performance Liquid Chromatograph

High-Performance Liquid Chromatograph

A high-performance liquid chromatograph (HPLC) measures the content of various components in a sample. A liquid medium flows through the HPLC flow channel. When a sample is introduced into the instrument, the components of the sample are separated in a column and the amount of each component is measured by a detector. HPLC can handle a much wider range of target samples and components than GC, including difficult-to-vaporize and heat-sensitive components.

For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

Top of This Page