Analysis of Mycotoxins
Mycotoxins are chemical products produced in fungus and are toxic to humans, animals, and crops. There are more than 300 known mycotoxins, including aflatoxin, deoxynivalenol, patulin, fumonisin and others, and some of them are strictly controlled under regulations. HPLC and LC-MS are mainly used for analysis of mycotoxins, and simultaneous analysis of multiple mycotoxins has been carried out for more efficient screening.
Simultaneous Analysis of 25 Mycotoxins (LC-MS/MS)
LC-MS/MS is effective for analysis of mycotoxins in complex matrices. Shown is an example of the simultaneous analysis of 25 mycotoxins by LC-MS/MS and the Method Package for Mycotoxins. All compounds, including positive and negative ions, were separated and detected with high sensitivity within 12 minutes. For analysis of mycotoxins with high sensitivity, eliminating carryover is indispensable. Modified rinse methods using the SIL-30AC autosampler can eliminate carryover of fumonisins.
Simultaneous Analysis of Mycotoxins (LC)
Simultaneous analysis of mycotoxins is important because multiple mycotoxins have been detected in a single food and may cause a synergistic effect. From this background, simultaneous analytical methods have been developed. Shown below are chromatograms of direct analysis of aflatoxins without any derivatization and rapid screening of 10 mycotoxins using HPLC.