Detection of Norovirus
Norovirus is a genus of viruses considered to be the principal cause of acute viral gastroenteritis. Norovirus infection is transmitted not only via oysters and other marine produce but also through contact with the feces or vomit of an infected person, and causes many food poisoning cases every year.
Norovirus positive samples were detected by the Microchip Electrophoresis System for DNA/RNA.
86 bp, 142 bp and 98 bp, 205 bp size RNA could be detected in Norovirus G1 and G2 samples, respectively.
* There are two Norovirus RNA types, G1 and G2.
DNA and RNA samples are separated by size by the electrophoresis system using a microchip to verify the size of nucleic acid (DNA/RNA) samples and conduct approximate quantitation. The microchip-based electrophoresis system quickly achieves electrophoresis separation, and a fluorescent detector ensures that analysis is conducted at high sensitivity and fully automatically.
Norovirus Detection Kit
Norovirus amplification is widely used for the detection of virus since it cannot be incubated. However, as biological samples contain many enzymes which powerfully catalyze RNA hydrolysis and interfere with the RT and PCR reactions, it is necessary to first separate the virus, and extract and purify its RNA prior to conducting Norovirus RNA gene amplification.
The Norovirus Detection Kit suppresses the action of RNA separation enzymes and RT and PCT reaction inhibiting enzymes in biological samples, and can amplify the Norovirus RNA so that it can be detected directly from the biological sample without the need for RNA purification.