Fast Analysis of Amino Acids Using the GC/MS Metabolite Component Database

Metabolite Analysis

Fast Analysis of Amino Acids Using the GC/MS Metabolite Component Database

The GC/MS Metabolite Component Database offers method files containing optimal column and consumables information, suitable analysis conditions and compound information, as well as libraries with retention indices. The metabolite database targets amino acids, fatty acids, and organic acids. It offers four types of mass spectrum library containing retention indices and mass spectra for these derivatives and four types of method file containing analysis conditions and data analysis conditions (Table 1).

Table 1 Method Files and Libraries Contained in the Database

The dedicated EZ: faast® derivatization pretreatment reagent kit can be used for amino acid analysis.

EZ: faast® contains reagents for amino acid pretreatment and derivatization, amino acid standard solutions (33 components), pretreatment equipment and an analysis capillary column.
Sample pretreatment and derivatization is completed in seven steps (taking approximately seven minutes). In addition to the 33 components, this kit supports over 50 amino acids and related compounds.
* EZ: faast® is manufactured by Phenomenex Inc.

Total Ion Chromatogram for Standard Solution of 33 Amino Acid Components

Metabolite Component Database

Retention indices, mass spectra, compound information (CAS numbers and structures), and method files for 311 metabolites (amino acids, fatty acids, and organic acids) are registered in the database.
Since retention indices are registered in the database, analysis is possible by simply analyzing an n-alkane standard mixture and using the GCMSsolution Ver.2.5 AART (Automatic Adjustment of Retention Time) function, which supports the identification of compounds when no standard sample is available by accurately predicting retention times.
The analysis methods registered in the database reduce the effort to determine analytical conditions and perform actual sample analysis.
The analytical methods contain the appropriate data analysis conditions.

For Research Use Only. Not for use in diagnostic procedures.

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