Identification of Microorganisms

Methods for identification of microorganism species include investigating the shape of the microorganism, biochemical examination, and the analysis of the 16S or 18S rDNA sequences. Each of these methods is complex, time consuming, and only captures one aspect of the microorganism species.
Identification of microorganisms by MALDI-TOF MS has attracted attention as a supplement to these methods. MALDI-TOF MS offers an overall measurement of microorganisms.
Such overall measurement is a new analytical method that captures the molecules in the microorganism by observing protein, peptide, and lipid ions.

Fig. 1 shows the direct measurement results for Escherichia coli NBRC 3972. The ions derived from the proteins and lipids in the microorganism are observed.

Fig. 1 MS Spectrum of Escherichia coli NBRC 3972

Fig. 2 shows the MS spectra of various bacteria. As the mass spectrum differs according to the bacteria, this method can be used to differentiate between bacteria.

Fig. 2 Comparison of MS Spectra for Bacteria Species

Laser Ionization Time-of-Flight Mass Spectrometer

AXIMA Performance
  • MS analysis possible by simply mixing the microorganism with the matrix solution. No sample pretreatment required.
  • Just 3 steps from sample preparation to measurement. Quick and easy measurement preparations.
  • Analysis complete in just two minutes after the start of measurements.
  • Permits high-throughput analysis (>1,000 samples/day).
  • No reagents required – low running costs

For Research Use Only. Not for use in diagnostic procedures.

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