Spectroscopic Properties - UV Absorption Spectrum -
UV Absorption Spectrum
Proteins such as antibody drugs generally exhibit a UV absorption peak near 280 nm. This absorption is due to the constituent amino acids tyrosine, tryptophan, and phenylalanine (aromatic amino acids). In some cases, it is necessary to determine the specific absorption (or molar absorption coefficient) of the target antibody drug. The specific absorption is determined by UV-VIS spectroscopy using a sample solution with a known protein concentration measured by amino acid composition analysis or the nitrogen quantitation method. The specific absorption of the target antibody drug is used for protein mass measurements by UV absorption.
The UV-1800 is a high-performance, compact, double-beam UV-VIS spectrophotometer. This compact, easy-to-use instrument achieves 1 nm resolution, which is the highest in the general-purpose instrument class.